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neonatal atcc pcs 200 012 experimental models  (ATCC)


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    Structured Review

    ATCC neonatal atcc pcs 200 012 experimental models
    Neonatal Atcc Pcs 200 012 Experimental Models, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atcc+pcs/Primary+Epidermal+Melanocytes%3B+Normal%2C+Human%2C+Neonatal/pm42276073-399-99-100
    Average 94 stars, based on 68 article reviews
    neonatal atcc pcs 200 012 experimental models - by Bioz Stars, 2026-09
    94/100 stars

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    Activity Assay:

    Article Title: Novel heterosteroids induce anabolic effects in human skeletal muscle cells: An integrated analysis of anabolic and catabolic signaling pathways.
    Article Snippet: The aim of this study was to investigate the anabolic potential of novel heterosteroids in human skeletal muscle cells.. A library of new heterosteroids was synthesized by selectively modifying the A and B rings of steroidal sapogenins (diosgenin, sarsasapogenin, and hecogenin), with six library members (S1, D3, D5, D7, D8 and D13) subsequently tested in vitro.. Compound selection was based on computational modeling intracellular signaling pathways regulating muscle hypertrophy and atrophy, coupled with pharmacological potential evaluation using cheminformatics tools.

    Cell Culture:

    Article Title: The multicomponent Passerini reaction as a means of accessing diversity in structure, activity and properties: Soft and hard vanilloid/cannabinoid modulators.
    Article Snippet: Primary adult epidermal keratinocytes were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) (ATCC®, PCS 200-011TM) and maintained as reported in Serafini et al., 2018 [52]. .. Briefly, cells were cultured in Dermal Cell Basal Media (ATCC, PCS 200− 030) supplemented with Keratinocyte Growth kit components (Bovine Pituitary Extract (BPE), recombinant tumor growth factor alpha (rhTGFα), L-glutamine, hydrocortisone hemi-succinate, insulin, epinephrine, and apotransferrin, (ATCC PCS 200-040), 10 units/mL penicillin and 100 μg/mL streptomycin (Sigma-Aldrich, St. Louis, MO)). .. Primary adult dermal fibroblasts were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) (ATCC®, PCS 200-012TM) and maintained as reported in Serafini et al., 2018 [52].

    Article Title: The multicomponent Passerini reaction as a means of accessing diversity in structure, activity and properties: Soft and hard vanilloid/cannabinoid modulators.
    Article Snippet: Primary adult dermal fibroblasts were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) (ATCC®, PCS 200-012TM) and maintained as reported in Serafini et al., 2018 [52]. .. Briefly, cells were cultured in Fibroblast Media supplemented with fibroblast growth kit-low serum, containing L-glutamine, hydrocortisone, hemi-succinate, recombinant fibroblast growth factor beta (rhFGFβ), rh-insulin, ascorbic acid, and FBS, (ATCC PCS-201- 041), 10 units/mL penicillin, and 100 μg/mL streptomycin (Sigma-Aldrich, St. Louis, MO). ..

    Recombinant:

    Article Title: The multicomponent Passerini reaction as a means of accessing diversity in structure, activity and properties: Soft and hard vanilloid/cannabinoid modulators.
    Article Snippet: Primary adult epidermal keratinocytes were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) (ATCC®, PCS 200-011TM) and maintained as reported in Serafini et al., 2018 [52]. .. Briefly, cells were cultured in Dermal Cell Basal Media (ATCC, PCS 200− 030) supplemented with Keratinocyte Growth kit components (Bovine Pituitary Extract (BPE), recombinant tumor growth factor alpha (rhTGFα), L-glutamine, hydrocortisone hemi-succinate, insulin, epinephrine, and apotransferrin, (ATCC PCS 200-040), 10 units/mL penicillin and 100 μg/mL streptomycin (Sigma-Aldrich, St. Louis, MO)). .. Primary adult dermal fibroblasts were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) (ATCC®, PCS 200-012TM) and maintained as reported in Serafini et al., 2018 [52].

    Article Title: The multicomponent Passerini reaction as a means of accessing diversity in structure, activity and properties: Soft and hard vanilloid/cannabinoid modulators.
    Article Snippet: Primary adult dermal fibroblasts were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) (ATCC®, PCS 200-012TM) and maintained as reported in Serafini et al., 2018 [52]. .. Briefly, cells were cultured in Fibroblast Media supplemented with fibroblast growth kit-low serum, containing L-glutamine, hydrocortisone, hemi-succinate, recombinant fibroblast growth factor beta (rhFGFβ), rh-insulin, ascorbic acid, and FBS, (ATCC PCS-201- 041), 10 units/mL penicillin, and 100 μg/mL streptomycin (Sigma-Aldrich, St. Louis, MO). ..



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    ATCC human epithelial cells
    Host cell damage and virulence capacity of mutants in sugar nucleotide biosynthesis. (A) Mutants grown in +/− 25 μg/ml Dox screened for <t>epithelial</t> damage using A-431 cells by LDH assay. The mean LDH released at 24 h post co-incubation is shown for repressed mutants (grown in presence of Dox; blue bars) and No-Dox controls (red bars). Red and blue horizontal lines indicate the mean LDH activity for wild type control (No-Dox) and wild type grown in presence of Dox respectively. Welsh t-test used for statistical analysis; error bars represent standard error of mean; p**** < 0.0001. (B) Survival plots of G. mellonella larvae infected with C. albicans mutants in: (I) GDP-mannose, (II) UDP-glucose and (III) UDP- N -acetylglucosamine biosynthesis in presence (solid lines) and absence (dotted lines) of Dox. No killing or improved survival was observed for a number of repressed mutants. No killing was observed in control larvae injected with equivalent volume of PBS. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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    Host cell damage and virulence capacity of mutants in sugar nucleotide biosynthesis. (A) Mutants grown in +/− 25 μg/ml Dox screened for epithelial damage using A-431 cells by LDH assay. The mean LDH released at 24 h post co-incubation is shown for repressed mutants (grown in presence of Dox; blue bars) and No-Dox controls (red bars). Red and blue horizontal lines indicate the mean LDH activity for wild type control (No-Dox) and wild type grown in presence of Dox respectively. Welsh t-test used for statistical analysis; error bars represent standard error of mean; p**** < 0.0001. (B) Survival plots of G. mellonella larvae infected with C. albicans mutants in: (I) GDP-mannose, (II) UDP-glucose and (III) UDP- N -acetylglucosamine biosynthesis in presence (solid lines) and absence (dotted lines) of Dox. No killing or improved survival was observed for a number of repressed mutants. No killing was observed in control larvae injected with equivalent volume of PBS. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Journal: The Cell Surface

    Article Title: Compromising UPD-sugar nucleotide biosynthesis attenuates Candida albicans viability, virulence and drug sensitivity

    doi: 10.1016/j.tcsw.2026.100170

    Figure Lengend Snippet: Host cell damage and virulence capacity of mutants in sugar nucleotide biosynthesis. (A) Mutants grown in +/− 25 μg/ml Dox screened for epithelial damage using A-431 cells by LDH assay. The mean LDH released at 24 h post co-incubation is shown for repressed mutants (grown in presence of Dox; blue bars) and No-Dox controls (red bars). Red and blue horizontal lines indicate the mean LDH activity for wild type control (No-Dox) and wild type grown in presence of Dox respectively. Welsh t-test used for statistical analysis; error bars represent standard error of mean; p**** < 0.0001. (B) Survival plots of G. mellonella larvae infected with C. albicans mutants in: (I) GDP-mannose, (II) UDP-glucose and (III) UDP- N -acetylglucosamine biosynthesis in presence (solid lines) and absence (dotted lines) of Dox. No killing or improved survival was observed for a number of repressed mutants. No killing was observed in control larvae injected with equivalent volume of PBS. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: Human epithelial cells derived from a vulvar squamous cell carcinoma (A-431 cell line; ATCC No.: CRL-1555) were cultured and maintained in DMEM medium supplemented with 10% ( v /v) heat inactivated foetal calf serum, 5% penicillin and 5% streptomycin.

    Techniques: Lactate Dehydrogenase Assay, Incubation, Activity Assay, Control, Infection, Injection